Tuesday, September 11, 2007
Characterization of an organ specific and pathogen responsive CC-NBS-LRR gene from Cotton (Gossypium hirsutum L.)
Mr. Baolong Zhang, Dr. Wanchao Ni, Ms. Yuwen Yang, and Dr. Xinlian Shen. Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences, Zhongling Street 50, Nanjing, China
Cotton diseases represent a major challenge to cotton growth. Cloning of cotton pathogen response gene and promoter was of great importance for us to improve disease resistance. In this study, a full length CC-NBS-LRR gene (GHNBS) and its 5´ flanking sequence have been cloned by race and tail PCR and further studied. The entire coding region is 2583bp and encodes a polypeptide of 861 amino acids with 28% maximum homology to an R gene of Arabidopsis deposited in the GenBank. Semi Quantitative RT-PCR showed GHNBS expressed in floral bud, petal, phloem, root and leaf and has a more expression pattern in root and leaf. The 5′ flanking sequence of GHNBS contained CAAT-box, TATA-box, several pathogen, SA, MeJA and ethylene responsive elements by PLACE analysis. Different 5′ promoter deletion derivatives with the coding region of the GUS gene fusions were transformed into Arabidopsis. Histochemical localization showed strong staining in roots, phenol of the stem and leave vein. GHNBS promoter was up regulated after SA,
See more of Genomics and Biotechnology Poster Session
See more of Genomics and Biotechnology
See more of The World Cotton Research Conference-4 (September 10-14, 2007)
See more of Genomics and Biotechnology
See more of The World Cotton Research Conference-4 (September 10-14, 2007)