Tuesday, September 11, 2007

Molecular Cloning and Expression Analysis of a Cys2/His2 type Zinc Finger Protein Gene in Upland Cotton

Ms. Yuwen Yang, Dr. Wanchao Ni, Mr. Baolong Zhang, and Dr. Xinlian Shen. Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences, Zhongling Street 50, Nanjing, China

The zinc finger proteins belong to the largest family of transcription factors. But there are few researches of Cys2/His2 type zinc finger proteins done in cotton and there is no submission of correlating ESTs to GenBank. In this study, a full length of one Cys2/His2 type zinc finger protein (GZFP) and its 5’ flanking sequence have been acquired by RT-PCR and tail PCR. The full length of the coding region is 804 bp and encodes a polypeptide of 268 amino acids with 40% homology to RBE protein of Arabidopsis deposited in the GenBank. It has the conserved zinc finger domain and the leucine rich region at the carboxyl terminus but no intron in the coding region. GZFP shows a more expression pattern in floral buds, ovaries, petals and roots than in phloem, xylem, fibers, leaves and seeds by RT-PCR. Analysis of the 5’ flanking sequence shows it contains several regulatory elements responsible for pollen and root expression, four core sites required for binding of Dof proteins and four light-regulated elements.

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See more of The World Cotton Research Conference-4 (September 10-14, 2007)