Tuesday, September 11, 2007
Construction and Analysis of SSH Library of Gossypium hirsutum L. upon Infection with Verticillium dahliae
Dr. Wanchao Ni, Mrs. Yuwen Yang, and Mr. Baolong Zhang. Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences, Zhongling Street 50, Nanjing, China
To obtain resistant-related candidate genes of Verticillium wilt from upland cotton, a high Verticillium wilt resistant cotton cultivar was chosen to be used for construction of a SSH library. Sequence similarity searches showed most of clones were homology to genes or ESTs from the SSH libraries induced by other pathogens. Their amino acid sequences were homology to some resistant-related genes, such as cytochrome P450 mono-oxygenase, Thaumatin-like Protein, chitinase and PR proteins. The full length of class III (Ghachi3) and class‡W (Ghachi4) chitinase was obtained by RACE and RT-PCR. The polypeptide encoded by these two genes shared 71% and 65% similarity to their homologous proteins of Arabidopsis. Ghachi3 were abundant in petals, buds and phloem, whereas Ghachi4 only showed an extremely high level in phloem by semi-quantitative RT-PCR. The accumulation of Ghachi4 increased remarkably after treatment of Verticillium dahliae and Fusarium oxysporum in the disease resistant cultivar exclusively. But Ghachi3 was also induced by Verticillium dahliae in the susceptible cultivar. In addition, the expression of both the two genes increased remarkably after treatment of
See more of Genomics and Biotechnology Poster Session
See more of Genomics and Biotechnology
See more of The World Cotton Research Conference-4 (September 10-14, 2007)
See more of Genomics and Biotechnology
See more of The World Cotton Research Conference-4 (September 10-14, 2007)