Tuesday, September 11, 2007
Expression profiles of Cry1Ac and Cry2Ab2 insecticidal proteins in tissues of Bollgard(r) and Bollgard II(r) in multiple Indian hybrid backgrounds
Dr. Anik L. Dhanaraj1, Mrs. Jayalekshmi Hariharan1, Dr. Anuradha Subramanian1, Dr. Subray P. Kamath1, Dr. Bindu Nair1, Dr. Komarlingam S. Mohan1, Dr. Ravi K. Channappa2, Dr. Sadananda A. Ramaiah2, Dr. Yelena A. Dudin3, Dr. Graham P. Head3, and Dr. Sakuntala Sivasupramaniam3. (1) Monsanto Research Center, 44/2A Vasant's Business Park, NH-7, Bellary Rd., Hebbal, Bangalore, India, (2) Mahyco Monsanto Biotec (India) Limited, Ahura Complex, 5th floor, # 96 Mahakali Caves Road, Andheri (E), Mumbai 400093, India, (3) Monsanto Company, 800 North Lindbergh Blvd, Mail Zone U3H, Creve Coeur, St. Louis, MO 63167
Monsanto cotton events MON531 (modified Cry1Ac gene) and MON15985 (Cry1Ac and Cry2Ab2 genes) have been introgressed into Gossypium hirsutum parental germplasm of 23 technology partners of Monsanto in India.
Since tissue contents of insecticidal proteins and bollworm tolerance are correlated, during the 2005 season, Cry1Ac concentrations in three tissues of 129 Bollgard™ hybrids were profiled (using quantitative ELISA) between 30 and 150 days after sowing. The tissue concentrations of Cry1Ac were adequate for the management of the three bollworms Viz., Helicoverpa armigera, Earias vittella and Pectinophora gossypiella. Likewise, the season-long tissue-concentrations of Cry1Ac and Cry2Ab2 proteins were independently profiled in tissues of 87 Bollgard II™ hybrids. The tissue contents of Cry1Ac in Bollgard and Bollgard II were essentially equivalent and the presence of Cry2Ab2 in Bollgard II hybrids increases bollworm control efficacy with IRM benefits.
The poster provides a glimpse of the season-long expression profiles of the two insecticidal proteins of the events MON531 and MON15985 in several Bollgard and Bollgard II hybrids across two growing seasons of 2005 and 2006.
Since tissue contents of insecticidal proteins and bollworm tolerance are correlated, during the 2005 season, Cry1Ac concentrations in three tissues of 129 Bollgard™ hybrids were profiled (using quantitative ELISA) between 30 and 150 days after sowing. The tissue concentrations of Cry1Ac were adequate for the management of the three bollworms Viz., Helicoverpa armigera, Earias vittella and Pectinophora gossypiella. Likewise, the season-long tissue-concentrations of Cry1Ac and Cry2Ab2 proteins were independently profiled in tissues of 87 Bollgard II™ hybrids. The tissue contents of Cry1Ac in Bollgard and Bollgard II were essentially equivalent and the presence of Cry2Ab2 in Bollgard II hybrids increases bollworm control efficacy with IRM benefits.
The poster provides a glimpse of the season-long expression profiles of the two insecticidal proteins of the events MON531 and MON15985 in several Bollgard and Bollgard II hybrids across two growing seasons of 2005 and 2006.
See more of Genomics and Biotechnology Poster Session
See more of Genomics and Biotechnology
See more of The World Cotton Research Conference-4 (September 10-14, 2007)
See more of Genomics and Biotechnology
See more of The World Cotton Research Conference-4 (September 10-14, 2007)